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Alpha Mannosidase - Specific Activity >3 U/mg；Activity >10 U/ml, high purity

Specific Activity ＞3 U/mg;Activity ＞10 U/ml

Features and benefits

Bioactivity: >10 U/ml

In stock

Item Number

A489971

Grouped product items
SKU	Size	Availability	Price	Qty
A489971-20μl	20μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$739.90
A489971-60μl	60μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,738.90

Basic Description

Product Name	Alpha Mannosidase - Specific Activity >3 U/mg；Activity >10 U/ml, high purity
Product Description	Product Description alpha-D-mannosidase, p-nitrophenyl-alpha-mannosidase, alpha-D-mannopyranosidase, alpha mannosidase, exo-alpha-mannosidase Alpha Mannosidase from Jack Bean cleaves α(1-2,3,6)-linked mannose. This enzyme is often used in conjunction with Core Alpha-(1-6) Mannosidase (E-AM02) if a noncleavable core α(1-6) mannose is present on the substrate. Specificty All Alpha-(1-2,3,6)-linked mannose Purity Each lot of α(1-2,3,6) mannosidase is tested for contaminating substances by incubating the enzyme for 24 hours at 37°C with substrates indicated in the table below. No detectable activity is evident for any of these potential contaminants. The detection limit of this assay is 5 μU/mL (IUB). For the protease assay, 10 μg of denatured BSA is incubated for 24 hours with 2 μl of enzyme. Analysis of the BSA band after SDS-PAGE should show no evidence of degradation. Molecular weight two polypeptides of 64,000 and 44,000 daltons Specific Activity Assay One unit of Alpha Mannosidase is defined as the amount of enzyme required to hydrolyze 1 µmole of p-nitrophenyl-alpha-p-mannoside to p-nitrophenol in 1 minute at pH 5.0 and 37˚C. Contents Alpha-(1-2,3,6) Mannosidase in 150 mM sodium phosphate, 0.1 mM ZnCl2 pH 7.5. (pH 7.5). Included with 20 µL and 60 µL pack sizes: 200 µl 5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0). Suggested usage 1. Add up to 1 nmol of oligosaccharide. 2. Add deionized water to 15 µl. 3. Add 4 µl 5x Reaction Buffer 5.0. 4. Add 1 µL of enzyme 5. Incubate ten minutes at 37˚C.
Specifications & Purity	Specific Activity ＞3 U/mg;Activity ＞10 U/ml
Bioactivity	>10 U/ml

Product Specifications

Concentration	Specific Activity ＞3 U/mg;Activity ＞10 U/ml
Storage Temp	Store at 2-8°C
Shipped In	Wet ice
Enzyme Commission Number	3.2.1.24

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Alpha Mannosidase - Specific Activity >3 U/mg；Activity >10 U/ml, high purity

Basic Description

AI Insight

Product Specifications

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Specifications

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

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