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Core Alpha Mannosidase - Specific Activity >1.5 U/mg；Activity 1 U/ml, high purity

Specific Activity ＞1.5 U/mg;Activity 1 U/ml

Features and benefits

Bioactivity: 1 U/ml

In stock

Item Number

C489984

Grouped product items
SKU	Size	Availability	Price	Qty
C489984-20μl	20μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$739.90
C489984-60μl	60μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,476.90

Basic Description

Product Name	Core Alpha Mannosidase - Specific Activity >1.5 U/mg；Activity 1 U/ml, high purity
Product Description	Product Description alpha-D-mannosidase, p-nitrophenyl-alpha-mannosidase, alpha-D-mannopyranosidase, core alpha mannosidase, exo-alpha-mannosidase The enzyme may inhibit other mannosidases, such as α(1-2,3,6) mannosidase, if a noncleavable α(1-6) mannose is present on the substrate. It should therefore always be added subsequent to digestion by other mannosidases. Alpha-(1-6) Core Mannosidase cleaves unbranched, terminal non-reducing mannose linked alpha-(1-6) to the mannosyl chitobiose core. Formulation The enzyme is provided as a sterile solution in 50 mM sodium phosphate pH 7.5, 0.1 mM ZnCl2. Contents Core Alpha Mannosidase in 50 mM sodium phosphate, 0.1 mM ZnCl (pH 7.5). 200 µl 5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0). Purity Each lot of α(1-6) mannosidase is tested for contaminating substances by incubating the enzyme for 24 hours at 37°C with substrates indicated in the table below. No detectable activity is evident for any of these potential contaminants. The detection limit of this assay is 5 μU/mL (IUB). The production host strain has been extensively tested and does not produce any detectable glycosidases. Molecular weight ~52,000 daltons Specific Activity Assay One unit of Alpha-(1-6) Mannosidase is defined as the amount of enzyme required to produce 2 µmoles of mannose in 1 minute at 37˚C, pH 5.0 from Alpha-(1-6) mannobiose. Suggested usage 1. Add up to 1 nmol of oligosaccharide. 2. Add deionized water to 15 µl. 3. Add 4 µl 5x Reaction Buffer 5.0. 4. Add 1 µL of enzyme 5. Incubate ten minutes at 37˚C. Stability Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity. Applications α(1-6) Core Mannosidase is useful for: Determining mannose linkages Rapidly removing the α(1-6) core mannose resistant to other mannosidases
Specifications & Purity	Specific Activity ＞1.5 U/mg;Activity 1 U/ml
Bioactivity	1 U/ml

Product Specifications

Concentration	Specific Activity ＞1.5 U/mg;Activity 1 U/ml
Storage Temp	Store at 2-8°C
Shipped In	Wet ice
Enzyme Commission Number	3.2.1.24

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Core Alpha Mannosidase - Specific Activity >1.5 U/mg；Activity 1 U/ml, high purity

Basic Description

AI Insight

Product Specifications

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Specifications

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

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