Call +1 (833) 552-7181 or Contact us Earn $25 in coupons on your first order with Aladdin!
Toggle Nav
My Cart
Search
Advanced Search
Menu
Account
Settings
྾
main product photo

Deoxyribonuclease I from bovine pancreas - ≥2,000 Kunitz units/mg dry weight, high purity , CAS No.9003-98-9

15 Citations
Features and benefits
In stock
Item Number
D128592
Grouped product items
SKU Size Availability Price Qty
D128592-25mg
25mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$330.90
D128592-100mg
100mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$893.90
D128592-500mg
500mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$3,022.90
Add to Wish List Compare
Bulk Order  DATASHEET
View related series
Click to view protein information: DNASE1

Basic Description

Product Name Deoxyribonuclease I from bovine pancreas - ≥2,000 Kunitz units/mg dry weight, high purity , CAS No.9003-98-9
Synonyms Deoxyribonucleate 5′-oligonucleotido-hydrolase;DNase I
Grade EnzymoPure™
Product Description

Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.
Specifications & Purity EnzymoPure™, ≥2,000 Kunitz units/mg dry weight
Biochemical and Physiological Mechanisms DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both

AI Insight

Product Specifications

Concentration ≥2,000 Kunitz units/mg dry weight
Storage Temp Store at 2-8°C
Shipped In Wet ice
CAS 9003-98-9
Enzyme Commission Number 3.1.21.1
Unit definition 1 Unit causes an increase in absorbance at 260nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0. Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at Aladdin. A

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

9 results found

Lot Number Certificate Type Date Item
K2306133 Certificate of Analysis Oct 25, 2023 D128592
K2301330 Certificate of Analysis Oct 25, 2023 D128592
L2024060 Certificate of Analysis Oct 25, 2023 D128592
L2024058 Certificate of Analysis Oct 25, 2023 D128592
K2203201 Certificate of Analysis Nov 12, 2022 D128592
H2205078 Certificate of Analysis Jul 08, 2022 D128592
H2205080 Certificate of Analysis Jul 08, 2022 D128592
H2205079 Certificate of Analysis Jul 08, 2022 D128592
I2414089 Certificate of Analysis Jul 08, 2022 D128592

Safety and Hazards(GHS)

WGK Germany 3
RTECS RF0750000

Specifications

 Specification Sheet

FAQs and Articles

FAQ
CRISPR/Cas9 and targeted genome editing
Categories: Technical articles
Tags:
Nuclease Cell and Molecular Biology Research Targeting efficiency Off target mutation
Experimental Guide of Sample Preparation for Flow Cytometry
Categories: Protocols
Tags:
Protein sample preparation Flow Cytometry Antibody FACS
CUT&RUN-seq protocol
Categories: Protocols
Tags:
Nuclease ChIP CUT&RUN ChIC DNA-protein interaction MNase Concanavalin A ConA magnetic beads
RNA Extraction and Reverse Transcription Protocol
Categories: Protocols
Tags:
RNA extraction Reverse transcription TRIzol Cellular RNA DNase RNase

Citations of This Product

How to Cite Aladdin Scientific Products?
1. Shaopeng Liu, Dan Peng, Shuohan He, Xuan Li, Yi Wu, Xin Liu, Yang Zhang, Peng Liu, Kaiyong Cai.  (2023)  Ternary low-temperature phototherapy nano-systems for the treatment of diabetic wounds.  Journal of Materials Chemistry B,  12  (1): (264-274).  [PMID:38088036] [10.1039/D3TB02224B]
2. Chenfei Han, Yu Xiao, Zhenjiang Liu, Daolin Du, Ming Li.  (2023)  Cascade amplifying aptasensor for positively correlated detecting OTA: Based on DNase I-assisted cyclic enzyme digestion and AgNPs@gel-enhanced fluorescence.  FOOD CONTROL,  153  (109970).  [PMID:] [10.1016/j.foodcont.2023.109970]
3. Ningning Lei, Xu Peng, Mengyue Hu, Chang Wan, Xixun Yu.  (2023)  Research on essential performance of oxidized chitosan-crosslinked acellular porcine aorta modified with bioactive SCPP/DOPA for esophageal scaffold with enhanced mechanical strength, biocompatibility and anti-inflammatory.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  241  (124522).  [PMID:37100332] [10.1016/j.ijbiomac.2023.124522]
4. Hui-Zhen Zhang, Mei Shu, Wen-Yu Yang, Hong Pan, Men-Xuan Tang, Yuan-Yang Zhao, Chan Zhong, Guo-Ping Wu.  (2023)  Isolation and characterization of a novel Salmonella bacteriophage JNwz02 capable of lysing Escherichia coli O157:H7 and its antibacterial application in foods.  LWT-FOOD SCIENCE AND TECHNOLOGY,  173  (114251).  [PMID:] [10.1016/j.lwt.2022.114251]
5. Luan Zhaohui, Liu Shuang, Wang Wei, Xu Kaige, Ye Shaosong, Dan Ruijue, Zhang Hong, Shu Zhenzhen, Wang Tongchuan, Fan Chaoqiang, Xing Malcolm, Yang Shiming.  (2022)  Aligned nanofibrous collagen membranes from fish swim bladder as a tough and acid-resistant suture for pH-regulated stomach perforation and tendon rupture.  Biomaterials Research,  26  (1): (1-20).  [PMID:36348451] [10.1186/s40824-022-00306-1]
6. Can Cheng, Xu Peng, Linjie Xi, Yihao Luo, Yuhang Wang, Yufan Zhou, Xixun Yu.  (2022)  Feasibility study of oxidized naringin as a novel crosslinking agent for crosslinking decellularized porcine Achilles tendon and its potential application for anterior cruciate ligament repair.  JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,  111  (2): (170-184).  [PMID:36054309] [10.1002/jbm.a.37440]
7. Guangquan Hu, Xiaojie Ding, Feng Gao, Jiehua Li.  (2022)  Calcium and integrin binding protein 1 (CIB1) induces myocardial fibrosis in myocardial infarction via regulating the PI3K/Akt pathway.  EXPERIMENTAL ANIMALS,      [PMID:34349085] [10.1538/expanim.21-0063]
8. Jiansheng Liu, Xueqin Qing, Qin Zhang, Ningyue Yu, Mengbin Ding, Zhaohui Li, Zhen Zhao, Zhiling Zhou, Jingchao Li.  (2021)  Oxygen-producing proenzyme hydrogels for photodynamic-mediated metastasis-inhibiting combinational therapy.  Journal of Materials Chemistry B,  9  (26): (5255-5263).  [PMID:34138994] [10.1039/D1TB01009C]
9. Yu Luo, Shenyu Huang, Lie Ma.  (2021)  A novel detergent-based decellularization combined with carbodiimide crosslinking for improving anti-calcification of bioprosthetic heart valve.  Biomedical Materials,  16  (4): (45022).  [PMID:33979785] [10.1088/1748-605X/ac0088]
10. Yu Luo, Lie Ma.  (2020)  Bioprosthetic heart valves with reduced immunogenic residuals using vacuum-assisted decellularization treatment.  Biomedical Materials,  15  (6): (65012).  [PMID:33016260] [10.1088/1748-605X/abaabf]
11. Yu Luo, Dong Lou, Lie Ma, Changyou Gao.  (2019)  Optimizing detergent concentration and processing time to balance the decellularization efficiency and properties of bioprosthetic heart valves.  JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,  107  (10): (2235-2243).  [PMID:31125175] [10.1002/jbm.a.36732]
12. Xuancheng Zhang, Zhaoyi Fang, Eunshinae Cho, Kai Huang, Jinzhong Zhao, Jia Jiang, Xiaoqiao Huangfu.  (2019)  Use of a Novel, Reinforced, Low Immunogenic, Porcine Small Intestine Submucosa Patch to Repair a Supraspinatus Tendon Defect in a Rabbit Model.  Biomed Research International,  2019  (9346567).  [PMID:31073531] [10.1155/2019/9346567]
13. Ma Liang, Guo Ting, Pan Shuli, Zhang Yuhao.  (2018)  A fluorometric aptasensor for patulin based on the use of magnetized graphene oxide and DNase I-assisted target recycling amplification.  MICROCHIMICA ACTA,  185  (10): (1-6).  [PMID:30276550] [10.1007/s00604-018-3023-z]
14. Yuanting Xu, Chengcheng Huang, Li Li, Xixun Yu, Xu Wang, Hong Peng, Zhipeng Gu, Yaping Wang.  (2013)  In vitro enzymatic degradation of a biological tissue fixed by alginate dialdehyde.  CARBOHYDRATE POLYMERS,  95  (148).  [PMID:23618251] [10.1016/j.carbpol.2013.03.021]
15. Yuanting Xu, Li Li, Hao Wang, Xixun Yu, Zhipeng Gu, Chengcheng Huang, Hong Peng.  (2013)  In vitro cytocompatibility evaluation of alginate dialdehyde for biological tissue fixation.  CARBOHYDRATE POLYMERS,  92  (448).  [PMID:23218319] [10.1016/j.carbpol.2012.09.096]

References

1. Shaopeng Liu, Dan Peng, Shuohan He, Xuan Li, Yi Wu, Xin Liu, Yang Zhang, Peng Liu, Kaiyong Cai.  (2023)  Ternary low-temperature phototherapy nano-systems for the treatment of diabetic wounds.  Journal of Materials Chemistry B,  12  (1): (264-274).  [PMID:38088036] [10.1039/D3TB02224B]
2. Chenfei Han, Yu Xiao, Zhenjiang Liu, Daolin Du, Ming Li.  (2023)  Cascade amplifying aptasensor for positively correlated detecting OTA: Based on DNase I-assisted cyclic enzyme digestion and AgNPs@gel-enhanced fluorescence.  FOOD CONTROL,  153  (109970).  [PMID:] [10.1016/j.foodcont.2023.109970]
3. Ningning Lei, Xu Peng, Mengyue Hu, Chang Wan, Xixun Yu.  (2023)  Research on essential performance of oxidized chitosan-crosslinked acellular porcine aorta modified with bioactive SCPP/DOPA for esophageal scaffold with enhanced mechanical strength, biocompatibility and anti-inflammatory.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  241  (124522).  [PMID:37100332] [10.1016/j.ijbiomac.2023.124522]
4. Hui-Zhen Zhang, Mei Shu, Wen-Yu Yang, Hong Pan, Men-Xuan Tang, Yuan-Yang Zhao, Chan Zhong, Guo-Ping Wu.  (2023)  Isolation and characterization of a novel Salmonella bacteriophage JNwz02 capable of lysing Escherichia coli O157:H7 and its antibacterial application in foods.  LWT-FOOD SCIENCE AND TECHNOLOGY,  173  (114251).  [PMID:] [10.1016/j.lwt.2022.114251]
5. Luan Zhaohui, Liu Shuang, Wang Wei, Xu Kaige, Ye Shaosong, Dan Ruijue, Zhang Hong, Shu Zhenzhen, Wang Tongchuan, Fan Chaoqiang, Xing Malcolm, Yang Shiming.  (2022)  Aligned nanofibrous collagen membranes from fish swim bladder as a tough and acid-resistant suture for pH-regulated stomach perforation and tendon rupture.  Biomaterials Research,  26  (1): (1-20).  [PMID:36348451] [10.1186/s40824-022-00306-1]
6. Can Cheng, Xu Peng, Linjie Xi, Yihao Luo, Yuhang Wang, Yufan Zhou, Xixun Yu.  (2022)  Feasibility study of oxidized naringin as a novel crosslinking agent for crosslinking decellularized porcine Achilles tendon and its potential application for anterior cruciate ligament repair.  JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,  111  (2): (170-184).  [PMID:36054309] [10.1002/jbm.a.37440]
7. Guangquan Hu, Xiaojie Ding, Feng Gao, Jiehua Li.  (2022)  Calcium and integrin binding protein 1 (CIB1) induces myocardial fibrosis in myocardial infarction via regulating the PI3K/Akt pathway.  EXPERIMENTAL ANIMALS,      [PMID:34349085] [10.1538/expanim.21-0063]
8. Jiansheng Liu, Xueqin Qing, Qin Zhang, Ningyue Yu, Mengbin Ding, Zhaohui Li, Zhen Zhao, Zhiling Zhou, Jingchao Li.  (2021)  Oxygen-producing proenzyme hydrogels for photodynamic-mediated metastasis-inhibiting combinational therapy.  Journal of Materials Chemistry B,  9  (26): (5255-5263).  [PMID:34138994] [10.1039/D1TB01009C]
9. Yu Luo, Shenyu Huang, Lie Ma.  (2021)  A novel detergent-based decellularization combined with carbodiimide crosslinking for improving anti-calcification of bioprosthetic heart valve.  Biomedical Materials,  16  (4): (45022).  [PMID:33979785] [10.1088/1748-605X/ac0088]
10. Yu Luo, Lie Ma.  (2020)  Bioprosthetic heart valves with reduced immunogenic residuals using vacuum-assisted decellularization treatment.  Biomedical Materials,  15  (6): (65012).  [PMID:33016260] [10.1088/1748-605X/abaabf]
11. Yu Luo, Dong Lou, Lie Ma, Changyou Gao.  (2019)  Optimizing detergent concentration and processing time to balance the decellularization efficiency and properties of bioprosthetic heart valves.  JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,  107  (10): (2235-2243).  [PMID:31125175] [10.1002/jbm.a.36732]
12. Xuancheng Zhang, Zhaoyi Fang, Eunshinae Cho, Kai Huang, Jinzhong Zhao, Jia Jiang, Xiaoqiao Huangfu.  (2019)  Use of a Novel, Reinforced, Low Immunogenic, Porcine Small Intestine Submucosa Patch to Repair a Supraspinatus Tendon Defect in a Rabbit Model.  Biomed Research International,  2019  (9346567).  [PMID:31073531] [10.1155/2019/9346567]
13. Ma Liang, Guo Ting, Pan Shuli, Zhang Yuhao.  (2018)  A fluorometric aptasensor for patulin based on the use of magnetized graphene oxide and DNase I-assisted target recycling amplification.  MICROCHIMICA ACTA,  185  (10): (1-6).  [PMID:30276550] [10.1007/s00604-018-3023-z]
14. Yuanting Xu, Chengcheng Huang, Li Li, Xixun Yu, Xu Wang, Hong Peng, Zhipeng Gu, Yaping Wang.  (2013)  In vitro enzymatic degradation of a biological tissue fixed by alginate dialdehyde.  CARBOHYDRATE POLYMERS,  95  (148).  [PMID:23618251] [10.1016/j.carbpol.2013.03.021]
15. Yuanting Xu, Li Li, Hao Wang, Xixun Yu, Zhipeng Gu, Chengcheng Huang, Hong Peng.  (2013)  In vitro cytocompatibility evaluation of alginate dialdehyde for biological tissue fixation.  CARBOHYDRATE POLYMERS,  92  (448).  [PMID:23218319] [10.1016/j.carbpol.2012.09.096]

Solution Calculators

Reviews

Customer Reviews

We have obtained your location, but we do not collect data on your location. Do we accept redirection to the corresponding region based on your location?

Privacy Policy Website Terms of Use Return policy Terms and Conditions of Sale Cookie Policy Sales Policy
Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.
Copyright © 2023-present Aladdin Scientific Corp . All rights reserved.