Does the flow-through antibody to CD206 need to be fixed to break the membrane?

Does the flow-through antibody to CD206 need to be fixed to break the membrane?

CD206 is a transmembrane protein that is expressed on the surface of cell membranes and intracellularly. Our anti-mouse CD206 flow antibody can bind to CD206 on the surface of the cell membrane and intracellularly, so it can be detected with or without membrane rupture, and you can choose whether to rupture the membrane or not according to the experimental design, and rupture detection is generally recommended.

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