IHC experiment, wondering what are the antigen repair conditions? Should I use EDTA or potassium citrate?

IHC experiment, wondering what are the antigen repair conditions? Should I use EDTA or potassium citrate?

Repair conditions are generally high temperature and high pressure or microwave heating. For example: antigen repair (high pressure method): add 10 mmol/ml citrate buffer (pH 6.0) to the pressure cooker (preparation: dissolve the antigen repair solution in the corresponding volume of ddH2O and mix well), heat to boiling, place the slices on the heat-resistant plastic slicing rack, put them into the cooker, cover the cooker, fasten the pressure valve, continue to heat, and set the holding pressure for 4 minutes, and then open the bleeder valve to release the gas when pressure is zero. After the time is up, open the bleeder valve to deflate, and open the lid of the pot to remove the inner pot and place it at room temperature to cool down, and take out the slices after the solution has cooled down to room temperature (about 30 min). Antigen repair (microwave method): add 10 mmol/ml of citrate buffer (pH 6.0) to the beaker (preparation: dissolve the antigen repair solution in the corresponding volume of ddH2O and mix), heat to boiling, place the slices in a heat-resistant plastic slicing rack, put them into the beaker, heat them for 30 min on medium-low heat and remove them from the beaker, leave them to cool down at room temperature, and take them out after cooling the solution to room temperature (about 30 min). Slicing (about 30 min). For the choice of IHC repair solution, please refer to the instruction manual of the antibody or the following protocols: for human samples, we recommend the use of EDTA repair solution at pH 9.0 (clinical samples); for rat/mouse samples, we recommend the use of citrate repair solution at pH 6.0 (research samples).