Experiments on Identification of Proprietary Chinese Medicines
The purpose of this experiment is to master the wolfberry chrysanthemum dihuang pill, heavenly king tonic pill traits identification characteristics; master the wolfberry chrysanthemum dihuang pill, heavenly king tonic pill microscopic, physicochemical characteristics and understanding of commonly used medicinal herbs, so as to do to see the drug well-known.
Operation method
microscopic observation
Materials and Instruments
QiJuDiHuang Pill Heavenly King Heart Mending Pill Move 1. Observe the microscopic identification characteristics of "Qiju Di Huang Wan". See Figure 1. For more product details, please visit Aladdin Scientific website.
Chloral Hydrate Glycerol Acetic Acid
Commonly used laboratory instruments for microscopic identification Biological microscope Alcohol lamp
[Prescription]: Lycium barbarum 40 g Chrysanthemum chrysanthemum 40 g Radix Rehmanniae Praeparata 160 g Cornu Cervi Pantotrichum 80 g Mudan Peony Peel 60 g Chinese yam 80 g Poria Cocos 60 g Zeligia 60 g
[Preparation: Crush the above eight flavors into fine powder, sieve and mix well. Each 100 g of powder with 35 ~ 50 g of refined honey and the appropriate amount of water, pan pills, dry, made of water honey pills; or add 80 ~ 110 g of refined honey to make small honey pills or large honey pills, that is, obtained.
[Microscopic identification]: Take this product and observe under microscope.
(1) Calcium oxalate needle crystal bundles existed in the mucus cells, starch granules triangular-ovate or rectangular, umbilical cord point short slit or herringbone shape. (Yam, Mudanpi, Zedoary)
(2) Irregularly branched masses colorless, solubilized by chloral hydrate; mycelium colorless or light brown. ( Poria cocos)
(3) Thin-walled tissue gray-brown to black-brown, cells crumpled, containing brown nuclei. (Radix Rehmanniae)
(4) Calcium oxalate clusters are present in colorless thin-walled cells, some of which are arranged in rows. (Peony bark)
(5) Epidermal cells of pericarp orange-yellow in color, polygonal in surface view, pericyclic wall slightly thickened with beads. (Cornus officinalis)
(6) Thin-walled cells roundish, with elliptic pores, integrated into groups of pores. (Cornus officinalis)
(7) Seed coat stony cells yellowish, wall undulate curved, cytosol cavity contains brown material. ( Lycium barbarum )
(8) The pollen grains are rounded, with spines on the outer wall and three germination holes. (Chrysanthemum)
2. Observe the microscopic characterization of "Tianwang Xinxinwan". See Fig. 2.
[Prescription]: Salvia miltiorrhiza 25 g Radix Codonopsis pilosulae 25 g Radix Angelicae Sinensis 50 g Acorus calamus 25 g Poria cocos 25 g Fructus schisandrae chinensis 50 g Ophiopogon flexuosus 50 g Asparagus ternatus 50 g Rhizoma Dioscoreae 200 g Radix et Rhizoma Glycyrrhiza glabra 25 g Polygonatum multiflorum (made from) 25 g Zaojubes indica (fried) 50 g Cortex Pseudostemma 50 g Platycodon Grandiflorum 25 g Glycyrrhiza glabra 25 g Rhizoma Cinnabaris 10 g
[Method]: The above sixteen flavors, cinnabar is flown into very fine powder; the remaining fifteen flavors of Salvia miltiorrhiza are crushed into fine powder, mixed with the above powder, sieved and mixed well. Every 100 g of powder with 20-30 g of refined honey, add the appropriate amount of water pan pills, dry, made of water honey pills; or add 50-70 g of refined honey into small honey pills or large honey pills, that is obtained.
[Microscopic identification]: Take this product and observe under the microscope.
(1) Irregularly branched mass colorless, dissolved by chloral hydrate; mycelium colorless or light brown. (Poria)
(2) Stone cells rhomboid or polygonal, one end slightly pointed, wall thicker, pores sparse. ( Codonopsis pilosula )
(3) Stone cells yellowish brown or colorless, oblong, rounded, or irregularly shaped, with obvious laminae. (Ginseng)
(4) Stone cells oblong or oblong, with very fine pores. (Asparagus)
(5) The epidermal stone cells of the testa are light yellowish brown, with a polygonal surface, thicker wall, fine pore grooves, and the cell cavities contain dark brown material. (Schisandra chinensis)
(6) Seed coat stone cells single scattered or 3 ~ 5 groups, light yellow, rounded polygonal, some one side protrudes, cell cavity contains calcium oxalate square crystal. ( Cypress kernel )
(7) The surface of cork cells is polygonal or rectangular, light yellow, containing reddish purple-brown pigment, dissolved by chloral hydrate. (Salvia miltiorrhiza)
(8) Surface view of cork cells: long polygonal or oblong-like, perforated perpendicular wall with interrupted shape, flat peripheral wall without texture. ( Polygonum multiflorum )
(9) Cork cells reddish brown or blackish brown, polygonal, wall thin. (Glycyrrhiza glabra)
(10) Associated milk ducts containing yellowish granular material. ( Codonopsis pilosula or Platycodon grandiflorus )
(11) Thin-walled tissue gray-brown to black-brown, cells much wrinkled, containing brown nuclei. (Dihuang)
(12) Oil cells round, containing yellow or yellowish brown oil. (Acorus calamus)
(13) Thin-walled cells around fiber bundles contain calcium oxalate square crystals, forming crystalline fibers. ( Glycyrrhiza glabra or Acorus calamus)
(14) The inner epidermal cells of the testa are brownish yellow, the surface is rectangular or square-like, and the peritubercular wall is bead-like thickened. (Ziziphi jujuba)
(15) Fusiform bast thin-walled cells yellowish, wall slightly thick, surface with obliquely interlaced fine reticulate texture, some with thin septa. (Angelica sinensis)
(16) Calcium oxalate needle crystals in bundles or scattered. (Ophiopogon or asparagus)
(17) Irregular fine granules dark brownish red, glossy, edges dark black. (Cinnabar)