Nucleic acid sequence retrieval experiments

Nucleic acid sequences are the starting point for understanding the structure, function, development and evolution of organisms. There are three authoritative international nucleic acid sequence databases, GenBank of the National Center for Biotechnology Information (NCBI) , EMBL-Bank of the European Molecular Biology Laboratory (EMBL for short), and DDBJ of the Genetics Research Institute of Japan . Source: Guangdong Pharmaceutical University Laboratory Manual.

Operation method

Nucleic acid sequence search

Principle

Master the operation of nucleic acid sequence search; Familiarize with GenBank database sequence format and the meaning of its main fields; Understand the EMBL database sequence format and the meaning of its main fields; Familiarize with the FASTA sequence format display and saving of GenBank database sequence format;

Materials and Instruments

Computer
Laptops Pens

Move

1. Use the Entrez information query system to search for the nucleic acid sequences BC060830 and NM_000230, link to extract the contents of the sequence, read the explanation of the sequence format, and understand its meaning;

2. GenBank database sequence format of FASTA sequence format display and save;

3. use SRS information query system to retrieve the nucleic acid sequence BC060830, connect to extract the content of the sequence, read the explanation of the sequence format and understand its meaning;

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