The greening effect of cytokinins on radish cotyledons
Cytokinin can promote cell division, delay aging, etc., so it is often used as a preservative for flowers, fruits and vegetables. Therefore, this experiment is mainly to learn and familiarize with the physiological role of cytokinin, and understand its significance in the storage and transportation of agricultural products.
Principle
Cytokinin can promote cell division, prevent the production of some hydrolytic enzymes such as nuclease and protease, thus making nucleic acids, proteins and chlorophyll less damaged, and at the same time, it has the effect of reducing the transportation of nutrients to the outside.
When isolated leaves of plants were placed in the appropriate concentration of cytokinin solution at 25-30°C under dark conditions, the decomposition rate of chlorophyll in the leaves was slower than that of the control, which proved that cytokinin has the effect of preserving green.
Operation method
The greening effect of cytokinins on radish cotyledons
Principle
Cytokinin can promote cell division, prevent the production of some hydrolytic enzymes such as nuclease and protease, thus making nucleic acids, proteins and chlorophyll less damaged, and at the same time, it has the effect of reducing the transportation of nutrients to the outside. Placing the isolated leaves of plants in the appropriate concentration of cytokinin solution and placing them in the dark at 25~30 °C, the decomposition of chlorophyll in the leaves was slower than that of the control, which proved that cytokinin has the effect of preserving green.
Materials and Instruments
1. Material: Radish seedlings with cotyledons just fully expanded. Move The basic process of cytokinin greening of radish cotyledons can be divided into the following steps: 1. 4 sets of Petri dishes were filled with 20 mL of 0, 5, 10, 20 μg- mL-1 of 6-BA solution respectively, and each concentration treatment was repeated twice. In each petri dish, 1 g of radish cotyledons with the same seedling age and growth were put into the petri dish, covered and incubated in a dark place with 25~30 pens. 2. After 1~2 days, the material was taken out and the solution on the cotyledons was blotted out with absorbent paper, and then the chlorophyll content (mg chlorophyll-g-1 fresh tissue) in the cotyledons of each treatment group was determined spectrophotometrically. Caveat 1. Plant material should be selected in such a way that it is homogeneous and the leaf segments in each treatment should come from the same leaves. 2. The pH of the prepared 6-phenylaminosilver (or zeatin) solution should be determined. For more product details, please visit Aladdin Scientific website.
2. Reagent: 0.1 mol - L
-1 HCl solution; 6-phenylaminosilver (or zeatin)
HCl solution; 6-phenylaminobufagin (or zeatin) solution: 10 mg of 6-phenylaminobufagin (6-BA) was weighed.
6-phenylaminobufotalin (6-BA) solution: 10 mg of 6-phenylaminobufotalin (6-BA) was weighed and diluted with 0.1 mol - L-1 HCl solution.
-1
HCl solution.
The concentration was 100 μg - mL-1 , and then diluted to 100 mL with distilled water.
-1
The concentration was 100 μg - mL-1 , and then diluted to 5, 10, 20 μg・mL-1 .
mL-1
and then diluted to 5, 10, 20 μg・mL -1.
The pH of the prepared solution was about 5. 3.
3. Apparatus: spectrophotometer, balance, measuring cylinder, small funnel, mortar, Petri dish, volumetric flask, scissors.