Blue native polyacrylamide gel electrophoresis (BN-PAGE) is an electrophoretic technique used to separate and analyze large protein complexes. It preserves protein complexes in their native state under non-denaturing conditions by treating samples with mild detergents and negatively charged dyes ...
The basic principles and operation steps of Western Blot experiments of histones are similar to those of other conventional proteins, both of which rely on specific antibodies to detect the presence and expression of target proteins. However, due to the special properties and functions of ...
When choosing a secondary antibody for western blotting, one of the main selection criteria is the species of the primary antibody that the secondary antibody binds to.