Most formalin-fixed tissues require an antigen retrieval step before immunohistochemical staining. Methylene bridges formed during fixation cross-link proteins and mask antigenic sites. Antigen retrieval methods break these methylene bridges and expose antigenic sites, allowing antibodies to ...
Choosing monoclonal or polyclonal antibodies? A single specific antibody produced by one type of B cell is called a monoclonal antibody. Monoclonal antibodies bind to a single specific antigenic determinant in a targeted manner, like a missile hitting a target with precision.
Immunohistochemistry (IHC) is a technique that uses the principle of specific binding between antigens and antibodies to locate, characterize and quantify antigens (peptides and proteins) within tissue cells by chemically reacting the marker antibody's color developer (fluorescein, enzyme, metal ...
