RNA dot blotting is a technique similar to Northern blotting; however, RNA samples cannot be separated by electrophoresis and require membranes for imaging .
In cells, proteins participate in extensive protein-protein interaction networks that appear as dynamic "protein machines" that assemble and disassemble in response to a constantly changing influx of intracellular, intercellular, and extracellular cues. An initial step in understanding protein ...
Western blotting is a technique that uses specific antibodies to separate proteins and is widely used to analyze the expression of specific proteins in cell or tissue extracts. This protocol details the steps for performing western blotting of cell culture and tissue samples using both ...
For western blotting, incubate the membrane with primary antibody diluted in 1×TBST (0.1% Tween®20) containing 5% w/v BSA or nonfat dry milk overnight at 4°C on a shaker. Please refer to the primary antibody product webpage for recommended primary antibody dilution buffer and antibody dilution.
