Tag Western blot

Articles by tag "Western blot"

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  1. Aladdin@Guidelines for the formulation of commonly used buffers for biological experiments In order to facilitate the experiments of scientific researchers, we have sorted out the buffer formulations commonly used in laboratory basic research in molecular biology, protein biology, microbiology and other aspects for researchers to facilitate access and use.
  2. Method and Precautions of SDS-PAGE Gel Preparation SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a commonly used technique to separate proteins based on size. By denaturating the protein and giving it a uniform negative charge, the protein migrates to the positive pole under the application of an electric field and is ...
  3. Principles of Western Blot Internal Reference Antibody Selection There are many kinds of Western Blot internal reference antibodies, such as β-actin, β-tubulin, GAPDH, Lamin B, etc., so how should we do the selection for our own experiments? Below is a brief introduction to the principles that should be followed in selecting internal reference antibodies.
  4. Histone Western Blot The basic principles and operation steps of Western Blot experiments of histones are similar to those of other conventional proteins, both of which rely on specific antibodies to detect the presence and expression of target proteins. However, due to the special properties and functions of ...
  5. Protocol of Western blot for Phosphorylated Proteins Phosphoprotein western blot is a widely used technique for detecting and analyzing the phosphorylation status of proteins, which is essential for understanding various biological processes and signal transduction pathways. Some common application scenarios include signal transduction research, ...
  6. What is the reason for the dirty background in the WB test? How can I fix it? The Western Blot experiment can be said to be the most basic experiment in the biology major. However, although WB experiments are done every day, a normal high-definition result picture is not something you can have whenever you want.
  7. Western Blot FAQ Analysis Western Blot FAQ Analysis
  8. Introduction to the principles related to the immunoblotting experiment Western blot is used to identify large molecular weight antigens (usually proteins) that can interact with specific antibodies and to determine the size of the antigen. Proteins are first separated by SDS-PAGE and then transferred by electrophoresis to a solid support, which includes ...
  9. Western experimental procedure Western experimental procedure
  10. General Immunoprecipitation Protocol Immunoprecipitation (IP) is a molecular biology technique based on antigen-antibody specific binding, which is used to selectively separate and enrich target proteins from complex biological samples. The core of this technology is to capture the protein of interest using specific antibodies on a ...
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