Bioactivity:Measured in a cell proliferation assay using B9 mouse hybridoma cells. The ED50 for this effect is 5.00-50.0 ng/mL. The specific activity of recombinant human IL-21 is >1.00 x 10^6 units/mg, which is calibrated against an internal reference standard for human IL-21.
Bioactivity:Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.300-2.60 ng/mL. The specific activity of recombinant human IL-15 is >2.00 x 10^8 units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554).
Bioactivity:Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 0.0300-0.250 ng/mL. The specific activity of recombinant human IL-2 is >5.00 x 10^6 IU/mg, which is calibrated against the human IL-2 WHO International Standard (NIBSC code: 86/500).
Bioactivity:Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 5-25 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. Optimal concentrations should be determined by each laboratory for each application.
Synonyms: MGC119418 | MGC119419 | MGC119420 | protein Wnt-3a | wingless-type MMTV integration site family, member 3A | Wnt3a | ...
Bioactivity:Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 1.50-12.0 ng/mL. The specific activity of recombinant human VEGF165 is > 8.0 x 10^5 units/mg, which is calibrated against the human VEGF165 WHO standard (NIBSC code: 02/286).
Bioactivity:Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25-100 pg/mL. The specific activity of recombinant human TNF-α GMP is >4.3 x 10^7 IU/mg, which is calibrated against the human TNF-α WHO International Standard (NIBSC code: 12/154).
Bioactivity:Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.1-0.4 μg/mL in the presence of 30 ng/mL of Recombinant Human BMP‑4
Bioactivity:Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.05-0.5 ng/mL. The specific activity of Recombinant Human Thrombopoietin is >1 x 10^7 units/mg, which is calibrated against the human Thrombopoietin reference standard (NIBSC code: 03/124).
Synonyms: Megakaryocyte colony-stimulating factor | Megakaryocyte growth and development factor | megakaryocyte stimulating fac...
Bioactivity:Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. The ED50 for this effect is 0.04-0.2 ng/mL.The specific activity of recombinant human TGF-β1 GMP is >1.0 x 10^7 units/mg, which is calibrated against the human TGF‑β1 Standard (NIBSC code: 89/514).
Bioactivity:Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.1-0.4 µg/mL.
Bioactivity:Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 1.00-8.00 ng/mL. The specific activity of recombinant human SCF is >1.0 x 10^6 units/mg, which is calibrated against the human SCF reference standard
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