Skip to the beginning of the images gallery

PKH 26 - ≥95.0%, high purity , CAS No.154214-55-8

≥95%

CAS#: 154214-55-8
Molecular Weight: 961.32

In stock

Item Number

P646619

Grouped product items
SKU	Size	Availability	Price
P646619-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$220.90
P646619-5mg	5mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$480.90
P646619-10mg	10mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$800.90

View related series

Fluorescent Dye (0)

Basic Description

Specifications & Purity	≥95%
Biochemical and Physiological Mechanisms	PKH 26 is a red fluorescent dye , PKH 26 can stably bind to the lipid region of cell membrane and emit red fluorescence (Ex/Em=551/567 nm), which is mainly used for in vitro cell labeling, in vitro cell proliferation studies and in vivo and in vitro cell
Storage Temp	Protected from light,Store at -20°C,Argon charged
Shipped In	Ice chest + Ice pads
Product Description	PKH 26 is a red fluorescent dye , PKH 26 can stably bind to the lipid region of cell membrane and emit red fluorescence (Ex/Em=551/567 nm), which is mainly used for in vitro cell labeling, in vitro cell proliferation studies and in vivo and in vitro cell tracing studies. In Vitro General Protocol 1.Preparation of PKH 26 working solution 1.1Preparation of the stock solution Dissolve 1 mg PKH 26 in 1 mL DMSO to obtain 1 mM of stock solution. Note: It is recommended to store the stock solution at -20°C and -80°C away from light and avoid repetitive freeze-thaw cycles. 1.2Preparation of PKH 26 working solution Dilute the stock solution in serum-free cell culture medium or PBS dilute at 1:50 or 1:100 to obtain 5-10 μM of working solution. Note: Please adjust the concentration of PKH 26 working solution according to the actual situation. 2.Cell staining 2.1 Suspension cells（6-well plate） a.Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10 6 /mL. b.Add 1 mL of working solution, and then incubate at room temperature for 10-45 minutes. c.Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant. d.Wash twice with PBS, 5 minutes each time. e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a. Culture adherent cells on sterile coverslips. b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes. d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry. Storage -80°C, 1 year Protect from light Precautions 1. Please adjust the concentration of PKH 26 working solution according to the actual situation. 2. This product is for R&D use only, not for drug, household, or other uses. 3. For your safety and health, please wear a lab coat and disposable gloves to operate. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Form:Viscous liquid

Names and Identifiers

Canonical SMILES	CC1(C)C(/C=C/C=C2N(CCCCCCCCCCCCCC)C3=C(C=CC=C3)C/2(C)C)=[N+](CCCCCCCCCCCCCCCCCCCCCC)C4=C1C=CC=C4.[I-]
Molecular Weight	961.32

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Chemical and Physical Properties

Solubility	DMSO : 1.67 mg/mL (1.74 mM; ultrasonic and warming and heat to 60°C)

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

PKH 26 - ≥95.0%, high purity , CAS No.154214-55-8

Basic Description

AI Insight