Recombinant Human CCL25 Protein, >97%(SDS-PAGE and HPLC), high purity

Features and benefits
  • Expression System: E.coli
  • Accession #: O15444
  • Protein Tag: No tag
  • Bioactivity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.
  • Endotoxin Concentration: <1.0 EU/μg
In stock
Item Number
rp143741
Grouped product items
SKU Size Availability Price Qty
rp143741-10μg
10μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$69.90
rp143741-50μg
50μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$299.90
rp143741-100μg
100μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$499.90
rp143741-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

Animal Free, >97%(SDS-PAGE and HPLC), Active, E.coli, No tag, 24-150 aa

Basic Description

Product Name Recombinant Human CCL25 Protein, >97%(SDS-PAGE and HPLC), high purity
Synonyms A130072A22Rik | C-C motif chemokine 25 | CCL 25 | CCL25 | CCL25_HUMAN | Chemokine (C C motif) ligand 25 | Chemokine (CC motif) ligand 25 | Chemokine C C motif ligand 25 | Chemokine CC motif ligand 25 | Chemokine TECK | Ck beta 15 | Ckb 15 | Ckb15 | MGC125
Grade ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description

Purity
>97% by SDS-PAGE and HPLC analyses.

Function
Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1.

Specifications & Purity ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE&HPLC)
Purity >97%(SDS-PAGE and HPLC)
Bioactivity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.
Endotoxin Concentration <1.0 EU/μg
Expression System E.coli
Species Human
Amino Acids 24-150 aa
Sequence MQGVFEDCCL AYHYPIGWAV LRRAWTYRIQ EVSGSCNLPA AIFYLPKRHR KVCGNPKSRE VQRAMKLLDA RNKVFAKLHH NTQTFQAGPH AVKKLSSGNS KLSSSKFSNP ISSSKRNVSL LISANSGL
Protein Tag No tag
Protein Length Full length protein
Carrier Free Yes
Animal Free Yes
Accession # O15444
Source Recombinant
Predicted molecular weight 14.3 kDa
SDS-PAGE 14.3 kDa

AI Insight

Images

Recombinant Human CCL25 Protein (rp143741)-SDS-PAGE
Recombinant Human CCL25 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining. Showing a single band at 15.5 kDa.

Recombinant Human CCL25 Protein (rp143741) - Protein Bioactivity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/mL.

Product Specifications

Shape Lyophilized
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconst

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ23F0700624 Certificate of Analysis Jul 21, 2023 rp143741
ZJ23F0700623 Certificate of Analysis Jul 20, 2023 rp143741
ZJ23F0700622 Certificate of Analysis Jul 20, 2023 rp143741

Specifications

References

1. Zaballos A, Gutiérrez J, Varona R, Ardavín C, Márquez G.  (1999)  Cutting edge: identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK..  J Immunol,  162  (10): (5671-5).  [PMID:10229797] [10.1021/op500134e]
2. Watts AO, Verkaar F, van der Lee MM, Timmerman CA, Kuijer M, van Offenbeek J, van Lith LH, Smit MJ, Leurs R, Zaman GJ et al..  (2013)  β-Arrestin recruitment and G protein signaling by the atypical human chemokine decoy receptor CCX-CKR..  J Biol Chem,  288  (10): (7169-81).  [PMID:23341447] [10.1021/op500134e]
3. Vicari, A P AP and 10 more authors..  (1997)  TECK: a novel CC chemokine specifically expressed by thymic dendritic cells and potentially involved in T cell development..  Immunity,      [PMID:9285413]
4. Grimwood, Jane J and 97 more authors..  (2004)  The DNA sequence and biology of human chromosome 19..  Nature,    (1):   [PMID:15057824]

Solution Calculators

Reviews

Customer Reviews

We have obtained your location, but we do not collect data on your location. Do we accept redirection to the corresponding region based on your location?