Basic Description

Product Name	Recombinant Human GM-CSF, CAS No.83869-56-1
Product Description	Product Describtion: GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant Human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acids, containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites. Initially reconstitute in water to 0.1-1.0 mg/ml. Store at 2°C to 8°C for up to 1 week or prepare for extended storage. After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20˚C to -80˚C. Accession Number: P04141
Specifications & Purity	≥98%
Bioactivity	The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
Endotoxin Concentration	<1.0 EU/μg
Amino Acids	18-144 aa
Sequence	AA Sequence:MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE
Accession #	P04141
Predicted molecular weight	14.6 kDa

AI Insight

Associated Targets(Human)

CSF2 Tbio Granulocyte-macrophage colony-stimulating factor (0 Activities)

Discover Target: CSF2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Product Specifications

Storage Temp	Store at -80°C
Shipped In	Ice chest + Ice pads
CAS	83869-56-1

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

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2 results found

Lot Number	Certificate Type	Date	Item
H2205200	Certificate of Analysis	Jul 27, 2022	G329704
H2205201	Certificate of Analysis	Jul 27, 2022	G329704

Specifications

Specification Sheet

FAQs and Articles

FAQ

BMDC Isolation Method

Categories: Protocols

Tags:

Cytokines GM-CSF BMDC Dendritic cells DC Bone marrow M-CSF RPMI

General Protocol for Western Blotting

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Western blotting WB Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Conjugated Secondary Antibodies HRP

Methods of Lysate Preparation for Western Blotting

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Western blotting SDS Cell lysis Tissue dissociation Protein extraction RIPA buffer

Western Blotting with Fluorescently Labeled Antibodies

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Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Fluorescently Labeled Antibodies Membrane Transfer

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SKU	Size	Availability	Price	Qty
G329704-5μg	5μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$490.90
G329704-20μg	20μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$1,764.90

Recombinant Human GM-CSF, CAS No.83869-56-1

Basic Description

AI Insight

Associated Targets(Human)

Product Specifications

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Specifications

FAQs and Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews