Recombinant Alkali-tolerant Protein A, CAS No.91932-69-5

Features and benefits
  • Expression System: E.coli
  • Endotoxin Concentration: <5.0 EU/mg
In stock
Item Number
rp192268
Grouped product items
SKU Size Availability Price Qty
rp192268-10mg
10mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$47.90
rp192268-50mg
50mg
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Production requires sourcing of materials. We appreciate your patience and understanding.
$369.90
rp192268-250mg
250mg
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Production requires sourcing of materials. We appreciate your patience and understanding.
$1,109.90
rp192268-1g
1g
Available within 8-12 weeks(?)
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$2,849.90

GMP, Monomer ≥90%(SEC-HPLC), Dimer ≤2.0%(SEC-HPLC), E. coli, No tag

Basic Description

Product Name Recombinant Alkali-tolerant Protein A, CAS No.91932-69-5
Synonyms Protein A | Staphylococcal Protein A | Staphylococcal Protein A Recombinant | Immunoglobulin G-binding protein A | gG-binding protein A | Immunoglobulin G binding protein A | SPA | rat-PA
Grade ActiBioPure™, Animal Free, Bioactive, Carrier Free, GMP
Product Description

This product is a freeze-dried powder of recombinant alkali resistant protein A expressed by Escherichia coli and purified by chromatography and freeze-drying. Aladdin recombinant protein A is a modification of the protein structure based on natural Staphylococcus aureus protein A. It consists of four Fc binding domain fragments linked together, with a molecular weight of 27KD. Compared with natural protein A, it removes the binding site with the immunoglobulin Fab region, enhances its binding ability with the Fc region, and improves the stability and alkali resistance of the protein. Recombinant protein A can be bonded to agarose gel to prepare an affinity filler for antibody purification. It can separate and purify antibodies of different subtypes of mammals or genetic engineering recombinant proteins containing Fc fragments of antibodies from ascites, serum, cell culture supernatant or cell extracts. The C-terminal of Aladdin recombinant alkali resistant protein A contains a cysteine, which can enhance its directional covalent bonding with gel.
2、Purpose
Biochemical research: used for immune analysis and purification of IgG. 
Preparation of affinity fillers for antibody purification: Multiple mammalian subtypes of antibodies or genetically engineered recombinant proteins containing antibody Fc fragments can be isolated and purified from ascites, serum, cell culture supernatant, or cell extracts.
3、Recommended usage
This product is a recombinant expression of alkali resistant protein A in Escherichia coli. Its C-terminus is designed with a cysteine residue, which can be coupled through the thiol non epoxy group on it to prepare affinity chromatography fillers. The activation of epoxy groups and protein A coupling methods need to be determined through experiments.

Specifications & Purity ActiBioPure™, Bioactive, GMP, Animal Free, Carrier Free, Monomer≥90%(SEC-HPLC), Dimer≤2.0%(SEC-HPLC)
Biochemical and Physiological Mechanisms Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immu
Endotoxin Concentration <5.0 EU/mg
Expression System E.coli
Predicted molecular weight 27 kDa
SDS-PAGE 24.3-29.7 kDa, under reducing conditions

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Product Specifications

Concentration Monomer≥90%(SEC-HPLC), Dimer≤2.0%(SEC-HPLC)
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
CAS 91932-69-5

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Specifications

Solution Calculators

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