Ribonuclease H (RNase H)

  • from Escherichia coli H 560 pol A1
Features and benefits
In stock
Item Number
R754998
Grouped product items
SKU Size Availability Price Qty
R754998-100U
100U
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$476.90

Basic Description

Product Name Ribonuclease H (RNase H)
Product Description

Nonspecific endoribonuclease that specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides.

Source: E. coli H560 pol A1
Storage Buffer: 25 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 50% glycerol (v/v), pH 8.0 (+4°C)
Volume Activity: 1 x 103 U/ml assayed according to Hillenbrand & Staudenbauer.
Ribonuclease H (RNase H) is a nonspecific endoribonuclease, localized to the nucleus and cytoplasm. It is ubiquitously found and widely present among many organisms including viruses and human.
Application:
Ribonuclease H (RNase H) has been used for:
  • In vivo RNA-primed initiation of DNA synthesis
  • Elimination of mRNA during second-strand cDNA synthesis
  • Site-specific cleavage of RNA
  • Detection of RNA:DNA regions in double-stranded DNA of natural origin
  • Removal of poly (A) sequences of mRNA if oligo (dT) is present
  • RNA extraction and quantitative reverse transcriptase polymerase chain reaction (RT-PCR)
Specifications & Purity from Escherichia coli H 560 pol A1
Biochemical and Physiological Mechanisms Ribonuclease H (RNase H) specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides. RNase H is associated with nucleic acid immunity. Usi

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Product Specifications

Concentration from Escherichia coli H 560 pol A1
Storage Temp Store at -20°C
Shipped In Ice chest + Ice pads
Stability And Storage Store at -15–-25 °C. (unopened)

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Specifications

Solution Calculators

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