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Vaccinia DNA Topoisomerase I - EnzymoPure™, This product is free from other DNA exonucleases, DNA endonucleases and RNases., high purity

EnzymoPure™

This product is free from other DNA exonucleases, DNA endonucleases and RNases.

In stock

Item Number

V752120

Grouped product items
SKU	Size	Availability	Price
V752120-200U	200U	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$100.90
V752120-1000U	1000U	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$414.90
V752120-5000U	5000U	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,657.90

Basic Description

Specifications & Purity	EnzymoPure™, This product is free from other DNA exonucleases, DNA endonucleases and RNases.
Stability And Storage	Store at -20℃.
Storage Temp	Store at -20°C
Shipped In	Ice chest + Ice pads
Grade	EnzymoPure™
Product Description	One unit of Vaccinia DNA Topoisomerase I catalyzes the conversion of 0.5µg of supercoiled pUC18 DNA to a relaxed state in 30 min at 37°C. Application： Vaccinia DNA Topoisomerase I can be used to relax the DNA supercoils for subsequent molecular biological reactions such as enzyme digestion, ligation between DNA vectors and PCR amplified fragments, nick repair and adapter ligation.Definition of activity: One unit of Vaccinia DNA Topoisomerase I catalyzes the conversion of 0.5µg of supercoiled pUC18 DNA to a relaxed state in 30 min at 37°C.10X Reaction Buffer: 500mM Tris-acetate, 1M NaCl, 25mM MgCl2, 1mM EDTA, pH 7.5 at 25°C.Enzyme storage buffer: 50mM Tris-HCl (pH 7.5), 1mM DTT, 0.1mM EDTA, 0.1M NaCl, 0.1% Triton X-100 and 50% (v/v) glycerolInactivation or inhibition: Vaccinia DNA Topoisomerase I can be inactivated by incubation at 80℃ for 20 minutes. Source： Recombinant Vaccinia Virus DNA Topoisomerase I expressed and purified from E. coli.Purity: This product is free from other DNA exonucleases, DNA endonucleases and RNases. Enzyme storage buffer： 50mM Tris-HCl (pH 7.5), 1mM DTT, 0.1mM EDTA, 0.1M NaCl, 0.1% Triton X-100 and 50% (v/v) glycerol Inactivation or inhibition： Vaccinia DNA Topoisomerase I can be inactivated by incubation at 80℃ for 20 minutes. Precautions： When supercoiled plasmids are treated with Vaccinia DNA Topoisomerase I, they should be separated on an agarose gel without ethidium bromide (EB). DNA staining with EB or other dyes must be performed after electrophoresis, as EB binds between the bases of DNA and catalyzes the formation of supercoils. After the electrophoresis is completed, the electrophoresis position of DNA fragments will not be changed even if the supercoil is formed again.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation. Instructions for Use： 1. Thaw all reagents required for the reaction. Mix well and place Vaccinia DNA Topoisomerase I on ice until use.2. Set up the reactions as follows:ComponentVolumeFinal ConcentrationNuclease-free Water15.9μl-10X Reaction Buffer2μl1XDNA (0.5μg/μl)2μl50ng/μlVaccinia DNA Topoisomerase I (10U/µl)0.1μl0.05U/µlTotal20μl-Note: If the enzyme concentration is too high, use the storage buffer provided in this product to dilute the enzyme appropriately before use.3. Transfer the reactions to a thermocycler or a water bath, incubate at 37℃ for 30 minutes, and then place them on ice.Note: When using a thermocycler for incubation, it is necessary to set the lid temperature at 40℃ to avoid enzyme inactivation.4. Perform electrophoresis with an agarose gel without EB, and stain the gel after electrophoresis for examination.

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Vaccinia DNA Topoisomerase I - EnzymoPure™, This product is free from other DNA exonucleases, DNA endonucleases and RNases., high purity

Basic Description

AI Insight

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Specifications

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

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