Immunoblotting using polyvinylidene difluoride (PVDF ) membranes is the most commonly used method for Edman sequencing. Protein samples are transferred from a 2
We provide background on multivariate analysis of proteomics data and also provide methods for data transformation of different data packages. This technique ca
Two-dimensional electrophoresis, as an important experimental tool for comparative proteomics studies, has been applied to conveniently reflect the differences
Staining of biphasic gel electrophoresis is a crucial step in comparative proteomics studies regarding the number of proteins to be measured, the accurate quant
Bidirectional gel electrophoresis and mass spectrometry for protein identification using fixed pH gradients (IPGs ) are currently the main techniques for proteo
Because of its outstanding ability to separate total proteins, bidirectional gel electrophoresis can be applied to the separation of all types of proteins. Howe
In all living cells the plasma membrane (PM ) exists as an interface between the cytoplasm and the environment and is one of the most complex and differentiated
The plant cell wall is a highly dynamic and chemically active component of the plant cell. The main components of the cell wall are polysaccharides, as well as
The integrity of the proteome of subcellular components such as the nucleus is largely determined by whether or not other cellular contaminants are removed from
The isolation and extraction of nuclear proteins has facilitated the study of plant proteomics. This technique relies on subcellular isolation with which the pr
