Identification of protein kinase substrates is a major focus of protein kinase research and provides evidence for resolving signal transduction pathways and the
The completion of the Arabidopsis and rice genome sequencing projects has greatly supported the application of proteomics methods such as protein microarray tec
A method has been developed for extracting intact proteins from SDS-PAGE gels and analyzing them by MALDI-TOF MS to determine their molecular mass. This method
High-resolution protein separation is an essential requirement for proteomics studies, and protein separation relies heavily on 2D IEF/SDS-PAGE; unfortunately,
Like other eukaryotic cells, glycosylation is one of the most studied post-translational events in plant cells. Depending on the type of connection between the
PROTICdb is a web-based database designed primarily to store and analyze plant proteomic data obtained by 2D polyacrylamide electrophoresis and mass spectrometr
Prior to the identification of membrane proteins by matrix-assisted laser-resolved time-of-flight mass spectrometry (MALDI-TOF-MS), these proteins need to be se
In this chapter, we present a detailed experimental method for the large-scale identification of proteins in rice leaf and root tissues using 2D liquid chromato
Tandem mass spectrometry is an effective technique for peptide characterization based on the fragmentation pattern of the peptide, also known as MS/MS scanning.
MALDI-TOF peptide mass fingerprinting (PMF) is a faster and more convenient method for identifying proteins. The genomes studied have been sequenced and annotat
