Articles by tag "DNA experiment"

1. DNA recombination in vitro
   posted this at Jun 5, 2025

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   DNA recombination, also known as gene cloning, is the most basic technique in gene manipulation and the core technology of molecular biology.DNA recombination r
2. Isolation of RNA based on size
   posted this at Jun 5, 2025

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   RNA samples may be denatured by treatment with formamide and by electrophoretic separation on gels containing formaldehyde, a method modified from Lehrachetal.
3. DNA electrophoresis (agarose gel)
   posted this at Jun 5, 2025

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   DNA electrophoresis can be used to (1) separate DNA fragments of different sizes, (2) identify target DNA fragments, and (3) purify and recover DNA fragments.
4. Poxvirus DNA extraction experiment
   posted this at Jun 5, 2025

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   If extracted poxvirus DNA is used for transfection, it should be isolated after proteinase K digestion and phenol extraction. See this experiment for specific m
5. Quantitative analysis of trace DNA by PCR method
   posted this at Jun 5, 2025

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   PCR is used to quantify the amount of a particular DNA sequence from 1 to 20,000 molecules per sample. In addition, it can assist in assessing the presence of c
6. Differential display PCR
   posted this at Jun 5, 2025

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   The source of this experiment is "Guide to Molecular Cloning Experiments, Third Edition", translated by Huang Peitang et al.
7. Synthesis of fixed-length single-stranded DNA probes from M13 phage templates
   posted this at Jun 5, 2025

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   The source of this experiment is "Guide to Molecular Cloning Experiments, Third Edition", translated by Huang Peitang et al.
8. Synthesis of non-fixed-length single-stranded DNA probes from M13 phage templates
   posted this at Jun 5, 2025

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   During synthesis, low concentrations of radiolabeled dNTP limit the length of probes to 200-300 nucleotides. However, newly synthesized DNA can be made 100-1000
9. Micronucleus assay for detecting DNA damage at the chromosome level
   posted this at Jun 5, 2025

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   The study of DNA damage at the chromosomal level is an important part of genotoxicology because chromosomal mutations are an important part of the cancer proces
10. Synthesis of cDNA probes from mRNA using random oligonucleotide primers
    posted this at Jun 5, 2025

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    In this protocol, poly(A)+ RNA is used as a template to synthesize cDNA probes by random priming reaction, and these probes can be used for differential screeni