This protocol describes the preparation of a deduction cDNA probe by hybridization with an mRNA driver followed by purification of single-stranded radiolabeled
The rise of biological barcoding demonstrates the active search for a system to identify eukaryotes based on the analysis of sequence diversity in short, standa
Not only is it easier to prepare specific single-stranded RNA probes than DNA probes, but they also generally produce stronger signals in hybridization reaction
The synthesis of cDNA in this protocol was carried out in four saturating concentrations of dNTP and one trace of radiolabeled dNTP. After deduction of hybridiz
Several methods exist to show changes in the expression levels of unknown genes in tissue samples in response to physiological stimuli. However, the morphologic
In recent years, DNA microarrays have been recognized as a standard method for molecular biology research. Especially in biomedical research, microarrays of com
This protocol removes the 5' phosphate of a nucleic acid by phosphatase, and then re-adds the phosphate to the nucleic acid in a radiolabeled form catalyzed by
In the forward reaction of T4 polynucleotide kinase, DNA substrates with flat ends, 5' concave ends or intramolecular cuts are less efficient than molecular lab
