The typical recovery rate of the RNA standard transcription system is lugRNA/ug plasmid DNA, which can be increased to 5~l0ug RNA/ug plasmid DNA by using the fo
Photochemical cross-linking technology is an effective means to study the interaction between RNA and protein in nuclear enameled egg complexes. The basic princ
The PAT method [ PCR poly(A) test ] can quickly and sensitively analyze specific mRNA from total RNA and quantitatively estimate the length of RNA poly(A) tails
Fe(II)-EDTA is capable of catalyzing the breakage of RNA or DNA and is a complementary method to RNase protection analysis. It has the advantage that there is l
In situ hybridization (ISH), also known as hybrid histochemistry or hybridization for cytology, is a technique that enables morphological demonstration of the p
The primer extension method can be used for RNA mapping and RNA 5' halo. In this method, an excess of a 5' end-labeled single-stranded RNA primer is hybridized
SI nuclease is an endonuclease that was isolated from Aspergillus oryzae. It can degrade single-stranded nucleic acids but not double-stranded nucleic acids. In
Spot and slit hybridization analysis of RNA enables the qualitative or semi-quantitative determination of RNA by directly spotting RNA samples onto nitrocellulo
Methylmercury hydroxide reacts with the imine bonds on uracil and guanine in RNA, which are involved in Watson-Crick base pairing. Therefore, methylmercury hydr
Under slightly acidic conditions, the two acetaldehyde groups of glyoxal interact with the imino group of guanosine to form a cyclic compound, which inhibits th
