Articles by tag "RNA Lab"

1. Nuclease cleaves RNA at a specific site
   posted this at Jun 5, 2025

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   Nuclease is a useful tool for obtaining site-specific cleavage products of long fragments of RNA for the study of RNA modification, for modification site analys
2. Ligation of RNA molecules with T4 RNA ligase
   posted this at Jun 5, 2025

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   T4RNA ligases have been used to generate many site-specific modified RNAs, especially oligonucleotide-modified and tRNA-modified with antisense codon tRNAs.In a
3. T4 DNA ligase ligates RNA molecules
   posted this at Jun 5, 2025

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   T4 DNA Ligase joins double-stranded complexes, including RNA-DNA heteroduplexes and RNA-RNA heteroduplexes, defectively.
4. RNA reverse transcription
   posted this at Jun 5, 2025

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   The RNA template is used to obtain DNA by reverse transcription, which is then used to replicate and infect cells.
5. RNA radiolabeling
   posted this at Jun 5, 2025

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   Intramolecular labeling of RNA during transcription, post-transcriptional labeling of the 5' end of RNA using T4 polynucleotide kinase and labeling of the 3' en
6. nuclear transcription analysis
   posted this at Jun 5, 2025

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   Nuclear runoff (runoff) analysis can be used to examine the transcriptional efficiency of specific genes in isolated cell nuclei. For runoff analysis, the nucle
7. A simple and effective transcription method to reduce heterogeneity at the 3' end of RNA
   posted this at Jun 5, 2025

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   Ribosyl C2'-methoxy modification of the nucleotides at the 2 5' ends. Amplification of DNA by PCR with modified primers still reduces N+l activity, and since PC
8. Synthesize 5' capped transcripts
   posted this at Jun 5, 2025

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   Some RNA transcripts require an m7 G(5')Ppp(5')G cap at the 5' end, and they have higher translational efficiency only in cell-free extracts or in African Xenop
9. Effect of different methods of treating tissues on the yield of extracted RNA
   posted this at Jun 5, 2025

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   RNA Laboratory Techniques Manual (Molecular Cloning - Laboratory Guide Series)
10. Rapid isolation of total RNA from mammalian cells
    posted this at Jun 5, 2025

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    SDS lyses the cells and extracts the total RNA by acid phenol graded extraction, which is a suitable method for treating cells with relatively low mRNA content