Nucleic acids are accurately quantified using spectrophotometry because this method does not destroy the structure and also allows for sample recovery. RNA has
The S30 family of extracts is divided into three categories: the E. coli S30 Extract System for in vitro translation of circular DNA, the E. coli S30 Extract Sy
The African Xenopus oocyte in vitro translation system is capable of sustained modification and processing of most exogenous mRNAs due to its ability to translo
The mRNA extracted from mammalian cells or produced by in vitro transcription of cloned DNA can be translated in cell-free extracts to synthesize proteins, whic
There are two strategies for in vitro experiments; one is to co-incubate a plasmid containing the target gene at the Po ly( A) locus with cell-free extracts to
Deoxyribonucleases are oligodeoxyribonucleotides consisting of about three dozen deoxyribonucleotides, which are easy to prepare and convenient to use, and are
