Rat liver was homogenized, the supernatant was centrifuged, and RNA was extracted with a mixture of phenol and m-cresol. rRNA and mRNA were separated in the pre
Formalin-fixed, paraffin-embedded tissues from the pathology department can be used for RNA expression analysis. RNA isolated from biopsy, surgical, and cadaver
Whole blood contains nucleated leukocytes, so it is easy to extract RNA from blood, but red blood cells are rich in ribonuclease, so it is difficult to isolate
Because some tissues (e.g., pancreas and uterus) contain high concentrations of endogenous RNAases, more care must be taken in purifying tissue-derived RNA.
RNA preparation by guanidine thiocyanate extraction and cesium chloride centrifugation is suitable for isolation of RNA from cells with difficult separation of
This method is a simple and effective way to isolate RNA from oocytes, fertilized eggs and fetal cells of frogs, sea urchins, periphyton, worms and flies.
The following method is a simple and effective method for purifying cytoplasmic RNA from tissue culture cells, which appeared at an earlier stage of RNA isolati
Viral RNA extraction can be (1) used for the study of RNA viral replication mechanisms, (2) used for reverse genetics studies, and (3) used for other studies in
After the mRNA of the target gene has been identified by in vitro cell-free translation, the first strand of cDNA can be synthesized in the steps described in t
